DNA sequences responsible for specificity of DNA packaging and phage growth interference of bacteriophages T3 and T7

Abstract

T3 and T7 phages package recombinant plasmids carrying DNA necessary for DNA packaging (the pac sequences) of T3 and T7, respectively. Packaging is specific between T3 and T7. The pac sequence has a bipartite structure, consisting of target sequences for processing of concatemeric DNA (pac C) and its left side flanking sequence containing a promoter for phage RNA polymerase (pac B). To determine the sequences responsible for the specificity of plasmid DNA packaging, plasmids chimeric for the pac B and pac C sequences of T3 and T7 were constructed. Analysis of packaging of the chimeric plasmid DNAs showed that pac B is responsible for the packaging specificity of T3 and T7 DNAs. Plasmids carrying the genetic right end of T3 and T7 DNA interfered with the growth of T3 and T7 phages, respectively. Interference was specific between T3 and T7. pac B and sequences between pac B and pac C, but not pac C, were responsible for the interference. The specificity of interference was determined by pac B and sequences responsible for interference were partially defined.