Study of bacteria Listeria monocytogenes in spontaneous aborted women in Salah Al-deen province

Abstract

This study was performed between January 2013 to the end of June 2013, involving 94 spontaneous aborted women at 15-45 years who attended TGH in Salah Al-deen province. They were examined and taking placental pits and cervical swabs. For diagnosis, used Gram staining, cultures, biochemical tests, API system and molecular technique including polymerase chain reaction (PCR). The spontaneous aborted women with Listeria infection was 13 (13.82%) out of 94 aborted women, while 81(81.86%) of aborted women had other causes of abortion. The most affected age group with Listeria infection was 25 – 34 years old represented 7 (53.84%) aborted women. The most infected patients from rural area was 61.53%, while in urban was 38.46%. The most aborted women with Listeria infection were had previous abortions within first trimester (less than 12 weeks of gestational age) were 8 (100%) cases. The pathogens isolated from placenta pits and cervical swabs was Listeria monocytogenes represented 11(37.93%) out of 29 isolated pathogens, while Listeria ivanovii was 2 (6.80%). PCR was used for detection of virulence associated  gene of  Listeria monocytogenes including hly A which had been found to be amplifying of their DNA fragments. The PCR products showed the eleven isolates of Listeria monocytogenes had the hly A gene