C-terminal dimerization of apo-cyclic AMP receptor protein validated in solution

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Abstract

Although cyclic AMP receptor protein (CRP) has long served as a typical example of effector-mediated protein allostery, mechanistic details into its regulation have been controversial due to discrepancy between the known crystal structure and NMR structure of apo-CRP. Here, we report that the recombinant protein corresponding to its C-terminal DNA-binding domain (CDD) forms a dimer. This result, together with structural information obtained in the present NMR study, is consistent with the previous crystal structure and validates its relevance also in solution. Therefore, our findings suggest that dissociation of the CDD may be critically involved in cAMP-induced allosteric activation of CRP.

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APA

Sim, D. W., Choi, J. W., Kim, J. H., Ryu, K. S., Kim, M., Yu, H. W., … Won, H. S. (2017). C-terminal dimerization of apo-cyclic AMP receptor protein validated in solution. FEBS Letters, 591(7), 1064–1070. https://doi.org/10.1002/1873-3468.12613

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