Biosensor Performance of Phenol Analysis Using Microbial Consortium of Bacillus sp. and Pseudomonas sp.

Abstract

Amperometry biosensors for phenol and its derivatives detection have been introduced using various approaches, such as single enzyme, bi-enzyme, and single microbe. However, the main problem of using an enzyme is its stability. Meanwhile, a single microbe for detecting phenol, like using Bacillus sp., is limited in sensitivity. A novel biosensor based on a microbial consortium of Pseudomonas sp. and Bacillus sp. mixture was immobilized on the working electrode part of the screen printed carbon electrode (SPCE). Both bacteria were able to produce biofilm. It could be applied for biosensing techniques. The electrocatalytic response towards phenol was evaluated using cyclic voltammetry between -1 to 1 V, with a scan rate of 100 mV/s. The result showed good linearity between 6.4 - 63.9 mg/L (R2 = 0.91 -0.98). The average sensitivity and limit of detection (LoD) were obtained at 0.95±0.22 μA/ppm and 19.71±10.26 ppm, respectively.