Creation of a contusion injury in rabbit skeletal muscle using a drop-mass technique

2Citations
Citations of this article
10Readers
Mendeley users who have this article in their library.

Abstract

This study reports our experience in developing a simple, minor injury. After reviewing the literature, a 'drop-mass' method was selected where a 201 g, elongated oval-shaped weight was dropped up to 15 times through a 1 m tube onto the left vastus lateralis of New Zealand white rabbits. To determine the extent of injury and degree of healing, biopsies were obtained six days after injury from the healing vastus lateralis of each animal. The tissue was fixed in formal saline, embedded in wax, cut and stained with haematoxylin and eosin (H&E) and phosphotungstic acid haematoxylin (PTAH) and examined by light microscopy (LM). The 'optimal' injury was created after seven drops, where quite severe, mild and moderately severe trauma was caused to muscle in the juxta-bone, mid and sub-dermal regions respectively. In each region, the muscle exhibited features of healing six days after injury. The 'drop-mass' technique appears to cause a contusion within a single muscle of at least three degrees of severity. This previously unreported observation is of particular importance to other researchers wishing to investigate contusion injury in other animal models. © 2013. The Authors.

Cite

CITATION STYLE

APA

Deane, M. N., Gregory, M., Mars, M., & Bester, L. (2013). Creation of a contusion injury in rabbit skeletal muscle using a drop-mass technique. Journal of the South African Veterinary Association, 84(1). https://doi.org/10.4102/jsava.v84i1.957

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free