Adenosine triphosphate (ATP) is a central metabolite that functions as the energy currency in a living cell. Therefore, visualizing cellular ATP dynamics provides the fundamental information necessary to understand the molecular events involving life phenomena. Live cell imaging technologies using fluorescence (FL)-based indicators have been developed to analyze the dynamics of various biological processes, such as intracellular ATP synthesis and consumption. However, the application of FL-based indicators to plant cells is limited due to the presence of strong chlorophyll autofluorescence, which drastically worsen the signal-to-noise ratio. The bioluminescent (BL) indicators that do not require excitation light could overcome this problem. In this chapter, we introduce a methodology to analyze ATP dynamics in plant cells using BL ATP indicators.
CITATION STYLE
Sato, R., & Masuda, S. (2022). Live Cell Imaging of ATP Dynamics in Plant Cells. In Methods in Molecular Biology (Vol. 2525, pp. 259–266). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2473-9_19
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