Molecular methods for the assessment of microbial biofilms

Abstract

In a vast majority of metabolically active strains results in negative culture even if the patient is infectious, the usage of molecular methods is one of the important tools for analyzing approximately 99% of strains causing microbial colonies directly on biofilms and also permits the information to be assembled on a protocol of treatment. This technique not only provides an authenticated source to understand the information of bacterial biofilm but also does the assessment of targeted biofilm interference strategies in vivo and marks in identification of mono or multi-species population of bacteria. Different molecular techniques like next-generation sequencing (NGS), polymerase chain reaction (PCR), hybridization and microarray technology are highly specific and sensitive, which enables the discovery of new concepts regarding the role of molecular methods for diagnosis of microorganisms in biofilms. This advancement is designed in order to detect the presence of the etiologic agents as well as used to design the protocol for antibiotics.