Abstract
The cytochrome P450 (CYP) family contains 57 enzymes in humans. The activity of CYPs against xenobiotics is a primary consideration in drug optimization efforts. Here we describe a series of bioluminescent assays that enable the rapid pro filing of CYP activity against compound collections. The assays employ a coupledenzyme format where fire fly luciferase is used to measure CYP enzyme activity through metabolism of pro-luciferase substrates. © Springer Science+Business Media New York 2013.
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Auld, D. S., Veith, H., & Cali, J. J. (2013). Bioluminescent assays for cytochrome P450 enzymes. Methods in Molecular Biology, 987, 1–9. https://doi.org/10.1007/978-1-62703-321-3_1
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