A consortium comprised of an engineered Escherichiacoli DH5α and a natural pentachlorophenol (PCP) degrader, Sphingobiumchlorophenolicum ATCC 39723, was assembled for degradation of hexachlorobenzene (HCB), a persistent organic pollutant. The engineered E. coli strain, harbouring a gene cassette (camA+camB+camC) that encodes the F87W/Y96F/L244A/V247L mutant of cytochrome P-450cam (CYP101), oxidised HCB to PCP. The resulting PCP was then further completely degraded by ATCC 39723. The results showed that almost 40 % of 4 μM HCB was degraded by the consortium at a rate of 0.033 nmol/mg (dry weight)/h over 24 h, accompanied by transient accumulation and immediate consumption of the intermediate PCP, detected by gas chromatography. In contrast, in the consortium comprised of Pseudomonasputida PaW340 harbouring camA+camB+camC and ATCC 39723, PCP accumulated in PaW340 cells but could not be further degraded, which may be due to a permeability barrier of Pseudomonas PaW340 for PCP transportation. The strategy of bacterial co-culture may provide an alternative approach for the bioremediation of HCB contamination.
CITATION STYLE
Yan, D. Z., Mao, L. Q., Li, C. Z., & Liu, J. (2015). Biodegradation of hexachlorobenzene by a constructed microbial consortium. World Journal of Microbiology and Biotechnology, 31(2), 371–377. https://doi.org/10.1007/s11274-014-1789-7
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