First report of Xiphinema brevicolle Lordello et Costa, 1961 (nematoda, longidoridae) in Japan

Abstract

Mixed populations of Xiphinema americanum-group species were detected from a root zone soil sample of Japanese holly, Ilex crenata, during a survey for plant-parasitic nematodes of commercial ornamental plant nurseries in Chiba Prefecture, Japan. From the result of the morphological study, the species were identifed as X. brevicolle and Xiphinema sp. Tis is the frst record of X. brevicolle in Japan. Morphometrics of X. brevicolle generally agree with those of the type specimens and the topotype specimens. Xiphinema sp. morphometrically resembles X. paramonovi except for tail length. Te mitochondrial COI region, the nuclear 18S rDNA and the nuclear large subunit rDNA D2/D3 region of the species were sequenced and compared in the molecular study. For the COI region, PCR primers were newly designed to obtain longer sequences, ca. 900 bp, than previously used. Sequence identities of COI, 18S and D2/D3 regions between these two populations were 84.0-84.1%, 99.9% and 98.1-98.2%, respectively. Phylogenetic analyses of maximum likelihood trees were carried out to compare genetic relationships among the group and some suggestions were made on the X. brevicolle-subgroup. © Hiromichi Sakai et al.