Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells

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Abstract

Background: Inherited and acquired retinal degenerations are frequent causes of visual impairment and photoreceptor cell replacement therapy may restore visual function to these individuals. To provide a source of new retinal neurons for cell based therapies, we developed methods to derive retinal progenitors from human ES cells. Methodology/Physical Findings: In this report we have used a similar method to direct induced pluripotent stem cells (iPS) from human fibroblasts to a retinal progenitor fate, competent to generate photoreceptors. We also found we could purify the photoreceptors derived from the iPS cells using fluorescence activated cell sorting (FACS) after labeling photoreceptors with a lentivirus driving GFP from the IRBP cis-regulatory sequences. Moreover, we found that when we transplanted the FACS purified iPSC derived photoreceptors, they were able to integrate into a normal mouse retina and express photoreceptor markers. Conclusions: This report provides evidence that enriched populations of human photoreceptors can be derived from iPS cells. © 2010 Lamba et al.

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Lamba, D. A., McUsic, A., Hirata, R. K., Wang, P. R., Russell, D., & Reh, T. A. (2010). Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells. PLoS ONE, 5(1). https://doi.org/10.1371/journal.pone.0008763

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