This chapter will provide a concise overview of the history of mammalian in vitro cell culture from its first descriptions through to the modern era. A specific focus will be adopted to understand when the importance of oxygen control emerged within the field, and we will discuss some of the earliest examples. A description of the common and state-of-the-art systems designed to provide an approximation of physiological normoxia will follow before finally describing its application in the emerging field of stem cell biology, specifically pluripotent stem cells and bone marrow-derived mesenchymal stem cells.
CITATION STYLE
Forsyth, N. R., Steeg, R., Ahmad, M., Al Zubaidi, M., Al-Jumaily, R., Merkhan, M., & Dale, T. (2018). Mimicking Physiological Oxygen in Cell Cultures (pp. 129–137). https://doi.org/10.1007/978-3-319-74854-2_8
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