The receptor for human endothelial differentiation gene-1 protein (EDG-1) was C-terminally tagged with green fluorescent protein and expressed in the methylotrophic yeast, Pichia pastoris. EDG-1 expression was driven by the highly inducible alcohol oxidase 1 promoter. Expression of EDG-1 recombinant protein was detected by Western blot analysis and confocal microscopy. The recombinant EDG-1 receptor protein was located in the plasma membrane. Radioligand binding assays demonstrated that the EDG-1 receptors expressed in Pichia pastoris have specific and saturation binding of 32P-labeled sphingosine 1-phosphate. © Springer Science+Business Media B.V. 2006.
CITATION STYLE
Yang, G., Liu, T., Peng, W., Sun, X., Zhang, H., Wu, C., & Shen, D. (2006). Expression and localization of recombinant human EDG-1 receptors in Pichia pastoris. Biotechnology Letters, 28(19), 1581–1586. https://doi.org/10.1007/s10529-006-9125-4
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