Molecular Identification and Phylogenic Analysis of Phosphate Solubilizing Bacteria Aneurinibacillus migulanus from Rhizosphere Imperata Cylindrica, Karst Citatah, Bandung Barat, Jawa Barat, Indonesia

Abstract

Phosphate converted from insoluble phosphate bonds (through secretion of organic acids) to P-inorganic bonds by phosphate solubilizing bacteria (PSB). Two highly potential PSB isolates, BR.5 and BR.7, were isolated from rhizosphere Imperata cylindrica, ex-mining limestone land in Karst Citatah, Province Jawa Barat, Indonesia. From the screening of P-solubilizing test results, BR.5 showed the highest clear zones in pikovskaya agar containing Ca3(PO4)2. The molecular methods to determine the species of bacteria are DNA extraction, DNA amplification by PCR, qualitative DNA testing by electrophoresis, and sequencing. The result of PCR results of DNA bands by electrophoresis, BR.5 has 1500 bp fragment length, 16S rRNA gene yielded on the phylogenic tree of Isolate BR. 5 has a similarity or relationship with the species Aneurinibacillus migulanus Isolate Am25 with a bootstrap value of 99%. Based on the genetic distance between Isolate BR.5 and Aneurinibacillus migulanus, Isolate Am25 has a distance value of 0.71%.