Chemical characterization and biological activities of ulvan extracted from Ulva fasciata (Chlorophyta)

Abstract

Ulvan is a sulfated heteropolysaccharide present in the cell wall of Ulva species with a unique structure and biological potential used in various fields. Chemical characterization was carried out to determine the structure of ulvan from Ulva fasciata Delile collected from Eastern Harbor, Alexandria coast, Egypt. Ulva contains 31.5% carbohydrate with a total ulvan content of 43.66% of total carbohydrate (13.75 g/100 g DW) and sulfate content of 20.45% of ulvan. FTIR spectrum presented signals of the sulfate ester (C–O–S) and sulfate groups (S=O), typical for ulvan. GC–MS revealed that ulvan was mainly composed of rhamnose and fucose. 1H-NMR spectra of ulvan showed identical behavior of monosaccharides nature with peaks characteristic of sulfated polysaccharides at 3.2–5.3 ppm region. Scanning electron micrographs (SEM) demonstrated amorphous architecture, and the sulfated nature of polysaccharides was emphasized by EDX analysis. The extracted ulvan showed significant antimicrobial activity against human and fish pathogens as well as antifouling bacteria with minimum inhibitory concentrations (MIC) of 8 µg/mL. The extracted ulvan exhibited potent antioxidant activity with a scavenging effect of 84.93% for 2,2-diphenyl-1-picrylhydrazy free radical (DPPH). Moreover, it showed anti-arthritic properties for the first time with a maximum inhibition of 86.04% with IC50 of 43.21%, indicating their potential value for the health and food industry.