The ever-increasing number of different miRNAs and their association with a vast number of cellular dysfunctions and diseases have initiated several groups to investigate miRNA maturation, which ultimately leads to down regulation of a target messenger RNA (mRNA) and its downstream product. A rapid, convenient, and reliable assay to detect the Dicer-mediated miRNA-maturation step may facilitate research in this field. Here we describe the in vitro detection of the Dicer-mediated miRNA maturation step using unmodified pre-miRNA and branched rolling circle amplification. © 2014 Springer Science+Business Media, New York.
CITATION STYLE
Neubacher, S., & Arenz, C. (2014). Detection of microRNA maturation using unmodified pre-microRNA and branched rolling circle amplification. Methods in Molecular Biology, 1095, 109–119. https://doi.org/10.1007/978-1-62703-703-7_9
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