Ultrasensitive label-free aptamer-based electronic detection

Abstract

(Figure Presented) The highs and lows of proteins: A dramatically amplified aptamer-based bioelectronic assay has been developed that allows the ultrasensitive measurement of thrombin down to the femtomolar level. This label-free electronic detection of thrombin takes advantage of the intrinsic electroactivity of a second aptamer with guanine bases, and the enormous amplification feature of the polymerase chain reaction (PCR, see picture). © 2007 Wiley-VCH Verlag GmbH & Co. KGaA.