The microinjection of mRNA that is transcribed and capped in vitro into fertilized eggs and embryos of Xenopus laevis provides a powerful means for discovering the function of proteins during early development. Proteins may be overexpressed for a gain-of-function effect or exogenous protein function may be compromised by the microinjection of mRNA encoding "dominant-negative" proteins. This methodology is particularly suited for the investigation of the regulation of the cell cycle, checkpoints, and apoptosis in early development.
CITATION STYLE
Sible, J. C., & Wroble, B. N. (2009). Expression of exogenous mRNA in Xenopus laevis embryos for the study of cell cycle regulation. Methods in Molecular Biology (Clifton, N.J.), 518, 1–15. https://doi.org/10.1007/978-1-59745-202-1_1
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