Yatakemycin, a novel antifungal antibiotic produced by Streptomyces sp. TP-A0356

Abstract

In the screening of novel antifungal compounds, yatakemycin was found in the culture broth of Streptomyces sp. TP-A0356. Yatakemycin was obtained by solvent extraction of the fermentation broth and chromatographic purification using ODS column and preparative HPLC. The structure of yatakemycin was elucidated by NMR and CID-MS/MS experiments as a novel antibiotic belonging to a family of CC-1065 and duocarmycins known to be DNA alkylating agents. Yatakemycin inhibited the growth of pathogenic fungi such as Aspergillus fumigatus and Candida albicans with the MIC values of 0.01-0.03 μg/ml, more potent than amphotericin B (MIC: 0.1-0.5 μg/ml) or itraconazole (MIC: 0.03-0.2 μg/ml). It also showed potent cytotoxicity against cancer cell lines with the IC50 of 0.01-0.3 μg/ml.