Optimization of Lipase-catalyzed Synthesis of Fatty Hydroxamic acids from Terminalia catappa L. Kernel oil

Abstract

Fatty hydroxamic acids (FHA) have been successfully synthesized from Terminalia catappa L. (local name: Ketapang) kernel oil by a one-step lipase catalyzed reaction. The use of immobilized lipase as a catalyst to synthesize FHA has several advantages such as a simple purification of product and environmentally friendly. In addition, it also allows the reaction to be carried out under mild conditions, which reduces the reaction's side products. The optimal reaction condition obtains were 40°C temperature reaction for 25 h with the ratio of ketapang seeds oil (g) : Hydroxilamin (mmol) is 1 : 2.861 mmol and the ratio of lipase enzyme (g) : Ketapang kernel oil (g) is 0,015 : 1. Amount of hydroxamic group in 1 gr of sample is 2.46 mol. Qualitative test of FHAs was carried out by color test and FTIR. Complex colors of the FHA with copper (II) and iron (III) are green and dark red, respectively. For FTIR analysis of FHAs group, various peak appeared such as C=O amide (1685 cm-1), O-H (3434 cm-1), N-H (3261,09 and 1568,64 cm-1) and C-N (939,91 cm-1).