Evaluation of a ultraviolet B Light Emitting Diode (LED) for producing Vitamin D3 in human skin

Abstract

Aim: A commercially available light emitting diode (LED) that transmitted narrow band ultraviolet B (UVB) radiation was evaluated for its efficacy and efficiency to produce Vitamin D3 in human skin. Materials and Methods: Human skin samples were obtained from surgical procedures. The LED had peak emission wavelength of 295 nm. Skin samples were exposed to the UVB-LED for varying times and then were analyzed by high-pressure liquid chromatography (HPLC) to determine the Vitamin D3 content. Results: There was a statistically significant time- and dosedependent increase in the percent of 7-dehydrocholesterol that was converted to Vitamin D3 in the skin type II samples; 1.3%±0.5, 2.3%±0.6 and 4.5%±1.67 after exposure to 0.75 (11.7 mJ/cm2), 1.5 (23.4 mJ/cm2) and 3 (46.8 mJ/cm2) minimal erythemal doses (MEDs), respectively. Conclusion: The UVB-LED was effective and efficient in generating Vitamin D3 in human skin, in vitro. The amount of Vitamin D3 production increased in a dose-dependent fashion with increased UVB energy. UVB-LEDs can be developed for devices that can efficiently produce Vitamin D3 in human skin.