The anti-bacterial effect of Punica granatum extracts against antibiotic resistant Pasteurella haemolytica

Abstract

Background: Continuous researches on drugs that solve the problem of growing number of resistant bacteria are important. Objectives: This research aims to investigate the effectiveness of anti-bacterial properties shown by the extracts prepared from different parts of Punica granatum L. (punicaceae), grown in Syria, against Pasteurella haemolytica, which are resistant to all studied antibiotics. Materials and Methods: A total number of 504 samples of dead sheep lungs were investigated for detection of P. haemolytica, using blood agar, blue methylene, and biochemical tests (oxidase, catalase, indole, urease). Different parts of P. granatum (pericarp, leaves, flowers, seeds) were extracted by water, absolute alcohol, and ether using soxhlet device and rotary vacuum evaporator. Antibiotic susceptibility testing for P. haemolytica by Kirby-Bauer disk diffusion method was conducted, then the extracts susceptibility test against P. haemolytica was conducted. Results: P. haemolytica has infected 20.63% of the total number of samples. The alcoholic extracts prepared from different parts of P. granatum showed the high antibacterial effectiveness, the pericarp extract was the best, whereas the water and ether petroleum extracts had no antibacterial effectiveness against resistant P. haemolytica. Conclusions: Ethanol extracts of P. granatum (pericarp, leaves, flowers, seeds) have antibacterial effects against P. haemolytica which has shown resistance to all studied antibiotics. © 2013, Ahvaz Jundishapur University of Medical Sciences; Published by Kowsar Corp.