Pyranonigrin L, a new antioxidant, produced by the hot spring-derived fungus penicillium adametzii BF-0003

Abstract

A new antioxidant, designated pyranonigrin L, was isolated from the culture materials of the hot spring-derived fungus Penicillium adametzii BF-0003 by solvent extraction, ODS column chromatography, and HPLC. Its planar structure was elucidated using various analytical techniques including UV, IR, and NMR spectroscopy and MS. Its absolute configuration was determined by a comparison of its circular dichroism (CD) spectrum with those of structurally related compounds. Pyranonigrin L was found to exhibit anti-oxidative activity with an EC50 value of 553 ?M.