Single-dose acute toxicity of THJ-2201 designer Cannabis drug: LD50 and hematological and histological changes in mice

Abstract

Background: This study aimed to assess the single-dose toxicity of THJ-2201 by hematological and histopathological evaluation of liver and kidney specimens in Swiss albino mice. The experimental protocol included oral treatment of mice with different doses (5, 50, 300, 2000 mg/kg body weight of THJ-2201) for 24 h. At the end of the treatment, blood samples had been drawn, and renal and hepatic tissues have been excised from the experimental mice groups for hematological and histological examinations. Results: Results showed that dose-dependent treatment with THJ-2201 leads to mild tremor clinical signs with low doses and photophobia (sensitivity to light) and even cessation of breathing as a potential cause of death with high doses in treated mice. Conclusion: The LD50 value of THJ-2201 was 822.20 mg/kg, which is considered as a chemical compound of low toxicity. Histological studies confirmed that liver and kidney toxicities have been manifested in the findings of congestion, necrosis, inflammation, and bleeding within the liver and to lesser extent in the kidneys.