Nesprins are a family of nuclear transmembrane proteins anchored via Sun proteins to the nuclear membrane. Analysis of nesprins during human muscle development revealed an increase in nesprin-1-giant during early myogenesis in vitro. During the transition from immature to mature muscle fibres in vivo, nesprin-2 partly replaced nesprin-1 at the nuclear envelope and short nesprin isoforms became dominant. Sun1 and Sun2 proteins remained unchanged during this fibre maturation. In emerin-negative skin fibroblasts, nesprin-2-giant was relocated from the nuclear envelope to the cytoplasm, not to the endoplasmic reticulum, while nesprin-1 remained at the nuclear envelope. In emerin-negative keratinocytes lacking nesprin-1, nesprin-2 remained at the nuclear envelope. HeLa cell nuclear envelopes lacked nesprin-1, which was the dominant form in myoblasts, while a novel 130-kD nesprin-2 isoform dominated Ntera-2 cells. The results suggest the possibility of isoform-specific and tissue-specific roles for nesprins in nuclear positioning. © 2010 Wiley-Liss, Inc.
CITATION STYLE
Randles, K. N., Lam, L. T., Sewry, C. A., Puckelwartz, M., Furling, D., Wehnert, M., … Morris, G. E. (2010). Nesprins, but not sun proteins, switch isoforms at the nuclear envelope during muscle development. Developmental Dynamics, 239(3), 998–1009. https://doi.org/10.1002/dvdy.22229
Mendeley helps you to discover research relevant for your work.