Analysis of d-β-aspartyl isomers at specific sites in proteins

Abstract

Recent studies have shown that biologically uncommon d-β-aspartic acid residues accumulate in specific proteins during the aging process. However, aspartyl residues are not racemized uniformly because d-Asp appears to be confined to particular sites in these proteins. We here describe the method to identify the specific sites of d-β-aspartic acids inversion in proteins.