Development of flow injection spectrophotometric method for 1-napthylthiourea using sodium nitrite and sulphanilic acid diazotization reaction

Abstract

A simple spectrophotometric method in conjunction with flow injection analysis is developed for the quantitative analysis of 1-napthylthiourea (ANTU). The reaction is based on the alkaline hydrolysis of ANTU to 1-naphthylamine at 30°C, coupled with diazotized sulphanilic acid resulting in 4-(sulphophenylazo)-1-naphthylamine which is monitored at 495 nm. The limit of detection (S/N = 3) is 0.01 mg/L with a sampling throughput of 45/h. The method is linear over the range of 0.05-120 mg/L (R2=0.9995,n=7), with calibration equation y = 0.031x-0.018 (y = absorbance; x = mg/L) and relative standard deviation (n=3) 1.8-3.6%. Experimental variables are optimised, and the interfering effects of different pesticides, organic compounds, cations, and anions at environmentally relevant concentrations are investigated. The analysis of ANTU in spiked water samples is extracted with solid phase procedure using disposable Sep-Pak C18 cartridges, and the recovery was 93 ± 1.9-110 ± 3.0%. The results of the proposed method are compared with the HPLC method by applying the F-test and paired Student t-test at 95% confidence level.