A custom-developed SPRi instrument for biosensor research

Abstract

In this paper we present a custom-developed, small, fixed-angle Surface Plasmon Resonance imaging (SPRi) instrument that enables the biochips to be prepared in an array format providing SPR information simultaneously on up to 100 active sites (spots). Besides the presentation of the hardware and software setup of our device we also demonstrate the application with pilot measurement results on different solutions containing NaCl, sucrose, avidin or DNA. The sensitivity of our instrument is calculated in function of the concentration. © 2011 Springer-Verlag Berlin Heidelberg.