The discovery of a delayed form of DXR-induced cardiotoxicity raises the question whether the presence of the drug or of metabolites in myocardial cells is necessary for the development of cardiotoxicity. The present investigations deal with a new method for the determination of DXR and of its main metabolite, DXR-3-ol, in cardiac cells. Rat hearts 'ex-vivo', isolated 24 h after i.v. administration of 6 mg/kg DXR and 20 μC of DXR-14C, were perfused with ice-cold Tyrode solution, which releases anthracyclines from extracellular spaces. After homogenization DXR and DXR-3-old were extracted from the cell at room temperature. The separation was carried out by HPLC; the recovery was about 95%, measured by the extracted radioactivity compared with that of the pellet residue.
CITATION STYLE
Rossini, L., Monti, E., Cova, D., & Piccinini, F. (1986). Determination of doxorubicin and doxorubicin-3-ol in rat heart. Archives of Toxicology, 59(SUPPL. 9), 474–478. https://doi.org/10.1007/978-3-642-71248-7_102
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