Plant diversity affects culturable soil bacteria in experimental grassland communities

Abstract

1 Utilization of carbon sources by culturable soil bacteria can be assessed with BIOLOG microtiter plates (contain 31 C sources). We used this technique to investigate bacterial community structure at various levels of plant diversity. Plant diversity levels were replicated and we investigated the influence of three plant functional groups, grasses, legumes and non-leguminous herbs, as well as the influence of individual plant species. 2 Catabolic activity and catabolic diversity of culturable soil bacteria were used to estimate their density (abundance) and functional diversity, respectively. Both increased linearly with the logarithm of plant species number and with the number of plant functional groups in experimental grassland ecosystems. These effects may have been caused by an increased diversity and quantity of material and energy flows to the soil. They may also have been mediated by increased diversity of soil microhabitats via a stimulation of the soil fauna. 3 The presence of particular plant species or functional groups in the different experimental communities stimulated the activity and functional diversity of the culturable soil bacteria in addition to their contribution via plant diversity. The legume Trifolium repens had the strongest effect and may be regarded as a keystone species with regard to plant-microbial interactions in the systems studied.