Lentiviral gene transfer technologies exploit the natural efficiency of viral transduction to integrate exogenous genes into mammalian cells. This provides a simple research tool for inducing transgene expression or endogenous gene knockdown in both dividing and nondividing cells. This chapter describes an improved protocol for polyethylenimine (PEI)-mediated multi-plasmid transfection and polyethylene glycol (PEG) precipitation to generate and concentrate lentiviral vectors.
CITATION STYLE
Larcombe, M. R., Manent, J., Chen, J., Mishra, K., Liu, X., & Nefzger, C. M. (2019). Production of high-titer lentiviral particles for stable genetic modification of mammalian cells. In Methods in Molecular Biology (Vol. 1940, pp. 47–61). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9086-3_4
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