Production of L-alanine from ammonium fumarate using two microbial cells immobilized with κ-carrageenan

Abstract

To produce L-alanine efficiently with immobilized microbial cells, the production system from ammonium fumarate by two-step enzyme reaction using aspartase activity of Escherichia coli cells and L-aspartate β- decarboxylase activity of Pseudomonas dacunhae cells in a single reactor was examined. It was found that the reaction should be started at pH around 8 and that the use of a mixture of immobilized E. coli cells and immobilized P. dacunhae cells was superior to that of co-immobilized E. coli-P. dacunhae cells. © 1985, The Society of Chemical Engineers, Japan. All rights reserved.