Statistical assessment of medium components by factorial design and surface methodology of L-Asparaginase production by isolated Streptomyces radiopugnans MS1 in submerged fermentation using Tapioca effluent

7Citations
Citations of this article
11Readers
Mendeley users who have this article in their library.

Abstract

Streptomyces radiopugnans MS1 was isolated from marine environment and identified as a potent strain for producing L-Asparaginase from Tapioca effluent an inexpensive substrate. To optimize the L-Asparaginase production of this bacterial strain, 23 fermentation variables were screened using a Plackett-Burman design and were then further optimized via response surface methodology based on a Box-/Behnken design. Four significant variables, i.e., Tapioca Effluent, Corn steep liquor, L-Aspaparagine and Areation, were selected. The optimum values of the tested variables by response surface methodology were; Tapioca Effluent, 5 (% v/v); Corn steep liquor, 2 (% w/v); L-Aspaparagine, 0.003 (% w/v); Aeration, 0.7 (vvm) found to be optimum for L-Asparaginase production. The experimental result (19.5 IU mL-1) in a medium optimized for L-Asparaginase production was in good agreement with the predicted value of a quadratic model (19.8 IU -1). A verification experiment was carried out to examine model validation and revealed more than 99% validity. The model was very satisfactory as the coefficient of determination was 0.99. L-Asparaginase production in the optimized medium (19.5±0.33 IU -1) increased 4.81-fold over that of the basal medium (4.05±0.84 IU -1) in the fermenter. © 2012 Knowledgia Review.

Cite

CITATION STYLE

APA

Senthil Kumar, M., Selvam, K., & Singaravel. (2012). Statistical assessment of medium components by factorial design and surface methodology of L-Asparaginase production by isolated Streptomyces radiopugnans MS1 in submerged fermentation using Tapioca effluent. Asian Journal of Applied Sciences, 5(5), 252–265. https://doi.org/10.3923/ajaps.2012.252.265

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free