Simultaneous determination of menthol and eucalyptol by the densitometric HPTLC method in some external analgesic formulations

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Abstract

Several analgesic pharmaceutical formulations dispensed for external use contain menthol and eucalyptol. Simultaneous determination of the two monoterpenes was achieved utilizing high-performance thin layer chromatography (HPTLC) densitometric analysis. The stationary phase composed of silica gel 60 F254 (E-Merck, Germany) glass-backed plates coated with 0.2 mm layers. The used mobile phase hexane: ethyl acetate 8: 2 (%, v/v) resulted in sharp, symmetrical and well-resolved peak at an Rf value of 0.34 ± 0.04 and 0.56 ± 0.04 for menthol and eucalyptol, respectively. Linearly range for menthol was 100-800 ng/spot (r2 = 0.9979), whereas that for eucalyptol was 52.25-1107 ng/spot (r2 = 0.9937). The developed method was validated following the International Conference on Harmonization guidelines. The method was found to be simple, accurate, time saving and enable analyses of many samples simultaneously.

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Alam, P., Alqasoumi, S. I., & Abdel-Kader, M. S. (2016). Simultaneous determination of menthol and eucalyptol by the densitometric HPTLC method in some external analgesic formulations. Journal of Chromatographic Science, 54(1), 58–63. https://doi.org/10.1093/chromsci/bmv075

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