Abstract
Metagenomics, shotgun metagenomics in particular, is a field that has disproportionately benefited from advances in high-throughput sequencing technologies. This chapter discusses the choice of sample number, depth, and sequencing technology, the shearing, size-selection, and multiplexing options on Illumina platforms, and outlines the steps in the bioinformatic analysis and sharing of metagenomic data.
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CITATION STYLE
Trimble, W. L., Greenwald, S. M., Owens, S., Glass, E. M., & Meyer, F. (2016). Metagenomic Design and Sequencing. In Field Guidelines for Genetic Experimental Designs in High-Throughput Sequencing (pp. 291–312). Springer International Publishing. https://doi.org/10.1007/978-3-319-31350-4_12
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