Isolation and Screening of Indigenous Fungus Producing Lignin Peroxidase from the Cocoa Plantation in Sepawon Kediri Regency Indonesia

Abstract

The aim of this research is to obtain indigenous fungus producing lignin peroxidase from the Cocoa Plantation in Sepawon Kediri Regency, Indonesia. Isolation was done using Minimum Salt Medium alkali lignin (MSM - L), purification was performed using monospore technique, lignin peroxidase activity was screened by agar medium containing methylene blue, and microscopic morphology of fungi was studied using slide culture technique. Five isolates of fungus were obtained and three isolates of fungus with different morphology were purified. The screening results showed that only two fungus isolates were indicated as the indigenous fungus producing lignin peroxidase were KLUM 1 and KLUM 2. KLUM 1 was morphologycally predicted as Sporotricum aereum and KLUM 2 was predicted close to genus Crysosporium sp.