Primary Dissociated Astrocyte and Neuron Co-culture

Abstract

The third edition of a collection of reproducible methods used in the neuroscience laboratory. The chapters carried over from the second edition have been extensively revised and updated, while 11 new chapters have been added which come from well-established tissue culture laboratories with proven records. The new chapters deal with preparation of substrata, use of serum-free media, maintaining hybridomas, and the production and purification of monoclonal antibodies. Also included are new and extensive chapters on the isolation, growth, and characterization of neural stem cells and glia progenitor cells.