Abstract
Trans-translation is a bacterial quality control system in protein synthesis facilitated by transfer-messenger RNA (tmRNA). Here, we describe the in vitro system using purified factors to evaluate the two steps of trans-translation: peptidyl-transfer from peptidyl-tRNA to alanyl-tmRNA and decoding of the resume codon on tmRNA. © 2012 Springer Science+Business Media, LLC.
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APA
Kurita, D., Muto, A., & Himeno, H. (2012). In vitro trans-translation assays. Methods in Molecular Biology, 905, 311–325. https://doi.org/10.1007/978-1-61779-949-5_20
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