Antigen arrays are fabricated using various antigens such as DNA, histones, synthetic peptides, recombinant proteins, or cell extracts to detect autoantibodies in autoimmune diseases, alloantibodies in transplantation, drug-induced antibodies or cancer-induced antibodies in blood or cell culture supernatant. In this protocol, we will provide a step-by-step executable procedure to perform antigen arrays, including antigen preparation and printing, blocking, sample loading, array detection, imaging, and data analysis.
CITATION STYLE
Yuan, Y., Wang, H., Lin, Z. T., Hong, X., Heon, M., & Wu, T. (2017). Protein arrays II: Antigen arrays. In Methods in Molecular Biology (Vol. 1654, pp. 271–277). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7231-9_20
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