Methanesulfonic acid ethyl ester (EMS) is effective in inducing mutants resistant to ampicillin, erythromycin, and butanol (15 g/l). Optimal mutagenesis occurs at 85-90% kill corresponding to a 15 minute exposure to 1.0% (v/v) EMS at 35 C. At optimal conditions, the frequency of resistant mutant CFU/ total CFU plated increases 100-200 fold. © 1985 Science and Technology Letters.
CITATION STYLE
Lemmel, S. A. (1985). Mutagenesis in Clostridiumacetobutylicum. Biotechnology Letters, 7(10), 711–716. https://doi.org/10.1007/BF01032281
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