Optimised synthesis of 6-iodoacetamidotetramethylrhodamine

Abstract

Practical improvements have been made to previous work on the synthesis of the title compound, particularly in the final step of converting its precursor chloroacetamide to the required iodoacetamide. Improvements include removal of contaminant(s) that inhibit the chloride to iodide exchange and better extraction procedures to minimise losses in work-up. The modified conditions for this step may be useful for synthesis of other thiol-reactive dyes where solubility of the dye is problematic. ©ARKAT.