MicroRNA-3666 inhibits breast cancer cell proliferation by targeting sirtuin 7

Abstract

The abnormal expression of microRNAs (miRNAs) is associated with cancer initiation and progression. miRNAs functioning as oncogenes or tumor suppressors represent novel biomarkers for cancer diagnosis, prognosis, and serve as therapeutic tools. MiR-3666 has been reported as a tumor suppressor in various types of cancer; however, its role in breast cancer remains unknown. In the current study, the aim was to investigate the potential role of miR-3666 in breast cancer. It was identified that miR-3666 was decreased in breast cancer cell lines and that the overexpression of miR-3666 inhibited breast cancer cell proliferation. Furthermore, miR-3666 promotes cell apoptosis of breast cancer cells. Bioinformatics analysis and dual-luciferase reporter assay demonstrated that miR-3666 targeted the 3'-untranslated region of sirtuin 7 (SIRT7) which was recognized as an oncogene. Overexpression of miR-3666 decreased SIRT7 expression levels, and knockdown of SIRT7 suppressed proliferation and promoted apoptosis of breast cancer cells. A rescue assay demonstrated that the restoration of SIRT7 expression markedly reversed the miR-3666-induced anti-tumor effects. Thus, the current study indicates that miR-3666 suppresses breast cancer cell proliferation by targeting SIRT7, and propose miR-3666 as a potential candidate for breast cancer therapy.