An Automated Multichannel Procedure for cDNA Microarray Image Processing

Abstract

In this paper, an automated multichannel procedure capable of processing cDNA microarray images is presented. Using a cascade of nonlinear filtering solutions based on robust order-statistics, the procedure removes both background and high-frequency corrupting noise, and correctly identifies edges and spots in cDNA microarray data. Since the method yields excellent performance by removing noise and enhancing spot location determination, the proposed set of cascade operations constitute the perfect tool for subsequent microarray analysis and gene expression tasks. © Springer-Verlag 2004.