Genotyping of recombinant Pichia pastoris strains

12Citations
Citations of this article
43Readers
Mendeley users who have this article in their library.

Abstract

A simplified amplified-fragment length polymorphism (AFLP) method was used to genotype Pichia pastoris strains obtained by transformation of P. pastoris strain GS115 with a single integration vector. A total of 14 transformants and 3 control strains were analyzed, which generated 16 different band patterns. A clonal variation was obtained after the transformation process due to genetic differences generated during the transformation event of the host strain. Furthermore, the cluster analysis showed that the transformants with lesser genetic differences with respect to the P. pastoris host strain are the recombinant strains with the highest level of recombinant protein production. © 2006 by the University of Wrocław.

Cite

CITATION STYLE

APA

Viader-Salvadó, J. M., Cab-Barrera, E. L., Galán-Wong, L. J., & Guerrero-Olazarán, M. (2006). Genotyping of recombinant Pichia pastoris strains. Cellular and Molecular Biology Letters, 11(3), 348–359. https://doi.org/10.2478/s11658-006-0029-z

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free