Novel Antihypertension Bioactive Compounds from Rusip

Abstract

Bioactive compounds from rusip has function as a antihypertension which inhibit Angiotensin Converting Enzyme (ACE). They was obtained by fractionation methode. Fractionation by molecular weight of rusip was to separate its bioactive compounds. Steps taken for this research was extraction and fractionation of rusip, assayed their ACE inhibition and peptide profile analysis. The results showed that there were four fractions from rusip extract, i.e F0 (free supernatant), F1 (Molecular wight fraction > 10 kD), F2 (Molecular wight fraction 1-10 kD), and F3 (Molecular wight fraction > 1 kD). There are three similar peptides in the Fo and F1 rusip fraction, i.e 44.78 kD, 25.90 kD and 17.18 kD, however there were no peptide in the F2 and F3 fractions. The ACE inhibitor activity of rusip fraction range for 34.40% to 97.52%, where F2 Fraction had highest ACE inhibitor activity. This research revealed those bioactive compounds which have highest activity of ACE inhibition from rusip was low molecular weight (under 10 kD) peptides or other biaoctives compounds.