Genetic polymorphism of cytochrome p450 (2C19) enzyme in iranian baluch ethnic group

Abstract

Interindividual differences in the metabolism of many drugs in humans are depended on genetic polymorphisms. The aim of this study was to determine the CYP2C19 genotype profiles of Baluch ethnic group by studying allelic differences and compare their frequencies with results of other studies in different populations. Polymerase Chain Reaction-Restriction Fragment Length Polymorphism technique was used for Genotyping of CYP2C19 gene. One hundred and forty unrelated healthy Baluch origin people who were referred to Health Center in Chahbahar, in Sistan and Baluchestan province enrolled in the study. The allele frequency of CYP2C19*1, CYP2C19*2 and CYP2C19*3 were 88.93%, 10% and 1.07%, respectively. 78.57% of subjects were with CYP2C19*1/*1 genotype. 20%, 0.71% and 0.71% subjects were with CYP2C19*1/*2, CYP2C19*1/*3 and CYP2C19*3/*3 genotypes, respectively. 0.71%, 20.71% and 78.57% of subjects were poor-, Intermediate- and extensive metabolisers. The allelic variants of CYP2C19*2 and CYP2C19*3 in Baluch ethnic group are comparable to other different population. CYP2C19*1 was the most frequently allele (88.93%) in our subjects. It is important to study the clinical significance of those variations for optimal drugs dosage which metabolize by the CYP2C19 enzymes in response to different substrates such as S-mephenytoin, methylphenobarbital, omeprazole, phenytoin, imipramine, proguanil, propranolol, and diazepam.