Toxicity Test with BSLT (Brine Shrimp Lethality Test) Method on Methanol, Ethyl Acetate Extract, Hexane on Seeds and Rind of Matoa extract (Pometia pinnata)

Abstract

Matoa seed and rind as an antioxidant for humans need to be strengthened by research on the toxicity. One of the toxicity tests is using the Brine Shrimp Lethality Test (BSLT) method. The mortality value was determined using a probit analysis to determine the toxicity value using the Lethal Concentration (LC-50) on the solvent fractions of ethyl acetate, methanol and hexane. The toxicity value (LC-50) of Matoa seed extract in hexane, ethyl acetate and methanol solvents were 153, 90 ppm, 141, 09 ppm and 153.65 ppm. The toxicity value (LC-50) of Matoa rind extract in hexane, ethyl acetate and methanol were 109, 34 ppm, 172, 98 ppm and 139.41 ppm, respectively. All the solvents had bioactive compound which had high toxicity because they have LC50 <1000 ppm . The highest toxicity was found in the rind of the matoa fruit extracted using hexane and methanol solvent with LC50 values 109, 34 ppm and 139.41 ppm, while the hexane extract had the lowest toxicity was 172.98 ppm.