Novel inhibitors of bacterial cytokinesis identified by a cell-based antibiotic screening assay

101Citations
Citations of this article
100Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

The continuous emergence of antibiotic resistance demands that novel classes of antibiotics continue to be developed. The division machinery of bacteria is an attractive target because it comprises seven or more essential proteins that are conserved almost throughout the bacteria but are absent from humans. We describe the development of a cell-based assay for inhibitors of cell division and its use to isolate a new inhibitor of FtsZ protein, a key player in the division machinery. Biochemical, cytological, and genetic data are presented that demonstrate that FtsZ is the specific target for the compound. We also describe the effects of more potent analogues of the original hit compound that act on important pathogens, again at the level of cell division. The assay and the compounds have the potential to provide novel antibiotics with no pool of pre-existing resistance. They have provided new insight into cytokinesis in bacteria and offer important reagents for further studies of the cell division machinery. © 2005 by The American Society for Biochemistry and Molecular Biology, Inc.

References Powered by Scopus

A simple statistical parameter for use in evaluation and validation of high throughput screening assays

5827Citations
N/AReaders
Get full text

Crystal structure of the bacterial cell-division protein FtsZ

746Citations
N/AReaders
Get full text

Structure, biochemistry and mechanism of action of glycopeptide antibiotics

591Citations
N/AReaders
Get full text

Cited by Powered by Scopus

The re-emergence of natural products for drug discovery in the genomics era

2014Citations
N/AReaders
Get full text

Bacterial cell division: Assembly, maintenance and disassembly of the Z ring

640Citations
N/AReaders
Get full text

Antituberculosis drugs: Ten years of research

505Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Stokes, N. R., Sievers, J., Barker, S., Bennett, J. M., Brown, D. R., Collins, I., … Errington, J. (2005). Novel inhibitors of bacterial cytokinesis identified by a cell-based antibiotic screening assay. Journal of Biological Chemistry, 280(48), 39709–39715. https://doi.org/10.1074/jbc.M506741200

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 37

55%

Researcher 19

28%

Professor / Associate Prof. 7

10%

Lecturer / Post doc 4

6%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 40

51%

Biochemistry, Genetics and Molecular Bi... 25

32%

Medicine and Dentistry 8

10%

Chemistry 5

6%

Save time finding and organizing research with Mendeley

Sign up for free