Preparation and diagnosis of xerogel nanocomposites and studying their effect on tnf-α level before and after loading dexamethason in male white rats induced rheumatoid arthritis

Abstract

The current study aimed to evaluate the mechanism of reducing the effect of (RA) after it was induced in male white rat with a Complete Freund’s Adjuvant (CFA) at TNF-α level by treating Xerogel Nanoparticles and dexamethasone nanocompounded (Xerogel / Dexa) . The free Xerogel nanoparticle had a surface roughness coefficient of 0.327 nm and the total percentage of volumes was 90% less than 130 nm, and the results also indicated that induced rheumatoid arthritis causes Significant increase (p <0.05) in the mean concentration of neoplastic necrosis factor (TNF-α) in the blood serum of male white rats, as they reached a concentration level (10.68 ± 461.80) Pg / ml in the positive control group (G2) for six weeks of treatment compared with the negative control group G1)), as the concentration level in it reached (69.97±0.88) Pg / ml for the same period, and there was also a significant decrease (P <0.05) in the level of concentration of TNF-α among members of the G5 group treated with the Xerogel / Dexa nanoparticle, as this concentration reached (182.98 ± 6.99 ) Pg / ml compared to the positive control groups (G2) treated with the free Xerogel (G3) and the group treated with free Dexa (G4) for a six-week treatment period in these groups as these concentrations reached (461.80± 10.68, 483.74± 3.59, 300.32± 4.03) Pg / ml, respectively.