The Invader assay (Third Wave Technologies) is a homogeneous, isothermal DNA probe-based method for sensitive detection of nucleic acid sequences. Invader reactions are performed directly on genomic DNA or total RNA targets; however, polymerase chain reaction- or reverse transcriptase polymerase chain re action-amplified products can also be used. Detection is achieved through target-specific signal amplification instead of target amplification. The assay is a highly accurate and specific detection method for both qualitative and quantitative analysis of single-nucleotide changes, insertions or deletions, gene copy number, infectious agents, and gene expression.
CITATION STYLE
Mast, A., & de Arruda, M. (2006). Invader assay for single-nucleotide polymorphism genotyping and gene copy number evaluation. Methods in Molecular Biology (Clifton, N.J.). https://doi.org/10.1385/1-59745-069-3:173
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